Journal: EMBO Reports

Article Title: Hyperfunction of post-synaptic density protein 95 promotes seizure response in early-stage aβ pathology

doi: 10.1038/s44319-024-00090-0

Figure Lengend Snippet: ( A ) Immunocytochemistry from WT primary cortical neuron cultures treated with Aβ 1-42 (Aβ42, 1 µM) or scrambled Aβ peptide (Scr, 1 µM) for 2 h at DIV 12–14 showing postsynaptic marker PSD-95 (red), presynaptic marker Synapsin-I (green), dendritic marker MAP2 (blue), and colocalization of PSD-95 and Synapsin-I (left). Quantification of colocalized synaptic puncta number (** P = 0.0069), PSD-95 puncta number (**** P < 0.0001), and Synapsin-I puncta number (*** P = 0.0001) (right). n = 14 cells from two independent cultures treated with Scr or Aβ42. ( B ) Immunocytochemistry from PSD-95 +/− (Het) primary cortical neuron cultures treated with Aβ42 (1 µM) or Scr (1 µM) for 2 h at DIV 12–14 showing postsynaptic marker PSD-95 (red), presynaptic marker Synapsin-I (green), dendritic marker MAP2 (blue), and colocalization of PSD-95 and Synapsin-I (left). Quantification of colocalized synaptic puncta number ( P = 0.8249), PSD-95 puncta number ( P = 0.302), and Synapsin-I puncta number ( P = 0.6488) (right). n = 12–15 cells from two independent cultures treated with Scr or Aβ42. ( C ) Immunocytochemistry from PSD-95 −/− (KO) primary cortical neuron cultures transduced with control lentivirus at DIV 9–10 and treated with Aβ42 or Scr (1 µM) for 2 h at DIV 12–14 showing postsynaptic marker Homer1b/c (red), presynaptic marker Synapsin-I (green), and colocalization of Homer1b/c and Synapsin-I (left). Quantification of colocalized synaptic puncta number ( P = 0.9962), Homer1b/c puncta number ( P = 0.5814), and Synapsin-I puncta number ( P = 0.4667) (right). n = 16–17 cells from two independent cultures treated with Scr or Aβ42. ( D ) Immunocytochemistry from PSD-95 −/− (KO) primary cortical neuron cultures transduced with PSD-95 lentivirus at DIV 9–10 and treated with Aβ42 or Scr (1 µM) for 2 h at DIV 12–14 showing postsynaptic marker Homer1b/c (red), presynaptic marker Synapsin-I (green), and colocalization of Homer1b/c and Synapsin-I (left). Quantification of colocalized synaptic puncta number (**** P < 0.0001) Homer1b/c puncta number (*** P = 0.0001), and Synapsin-I puncta number (*** P = 0.0009) (right). n = 16–17 cells from two independent cultures treated with Scr or Aβ42. Data Information: significance was determined by Student’s t test ( A – C ) or Mann–Whitney U test ( D ). Data are represented as mean ± SEM with * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns: non-significant. Scale bar: 5 μm. .

Article Snippet: Rabbit polyclonal anti-Synapsin-I antibody (ab8), chicken polyclonal anti‐Microtubule‐associated protein 2 antibody (MAP2, #ab92434), and rabbit anti-GluA1-C-terminus antibody (#Ab31232) are from Abcam.

Techniques: Immunocytochemistry, Marker, Transduction, MANN-WHITNEY

Journal: EMBO Reports

Article Title: Hyperfunction of post-synaptic density protein 95 promotes seizure response in early-stage aβ pathology

doi: 10.1038/s44319-024-00090-0

Figure Lengend Snippet: Quantification of Synapsin-I and representative western blots from WT primary cortical neuron cultures treated with amyloid-beta 1–42 (Aβ42; 1 µM) or scrambled Aβ peptide (Scr, 1 µM) for 2 h at DIV 12–14. n = 8 and 7 for Scr and Aβ42 groups, respectively. Significance was determined by Student’s t test ( P = 0.3604). Data are represented as mean ± SEM with ns: non-significant. .

Article Snippet: Rabbit polyclonal anti-Synapsin-I antibody (ab8), chicken polyclonal anti‐Microtubule‐associated protein 2 antibody (MAP2, #ab92434), and rabbit anti-GluA1-C-terminus antibody (#Ab31232) are from Abcam.

Techniques: Western Blot